Chromatography is widely perceived as an essential analytical tool due to its sensitivity and effectiveness in separating and identifying various mixtures of components. It has numerous applications in chemical and biological fields. The method is a technique for separating and analyzing components (solutes) of a mixture according to the relative amounts of compounds distributed between a moving fluid (mobile phase) and a stationary phase. The separation of compounds will happened by allowing a solution or mixture to seep through an adsorbent. The mobile phase in Chromatography may be either a liquid or a gas and based on the mobile phase it could be divided to the following subdivides:
The Mass Spectroscopy is an analytical method which is based on separation of ionic fragments of a component according to mass-to-charge ratios (m/z) in an electric or magnetic field.
In these systems the analyte is passed through an ionic source where a component is changed to ionic fragments by bombarding molecules with a stream of electrons under extremely low pressure. The stream of ionic fragments that differ in mass are accelerated into an electric or magnetic field which is used to change fragments paths. The detect measures the intensity of the each ionic beam and produce a pattern (mass spectrum). This spectrum can be stored in a computer to be compared with entries in a mass spectrum library and identify the target. Mass Spectroscopy is a very specific identifying technique specially when coupled with Gas or Liquid chromatography.