The LC/MS system is coupling of liquid chromatography and mass spectrometry which may be used to provide information about the molecular weight, structure, identity and quantity of a specific component in a sample matrix. Combination of LC with amazing separating power with supreme detection of mass spectrometry makes the technique one of the desirable analyzing tools. LC/MS is quite new developed method which had a great influence on protein and peptide biochemistry.
Liquid Chromatography /tandem mass spectrometry (LC-MS/MS)
The main characteristics of LC-MS/MS technology are high selectivity, sensitivity, and throughput which cause growingly use of technique in the clinical laboratories. The LC-MS/MS system added a second phase of mass fragmentation. This enable user to program the detector and select certain ions to fragment which help to eliminate high matrix backgrounds effects. the is an important technique study purity and impurity profiles in drugs , pharmacokinetic studies, metabolites identification in the plasma and urine, doping analysis, toxicological studies and so on.